A variable temperature magnetic susceptibility measurement of a crystalline sample of 3 was done on a mpms 3 quantum design squid magnetometer. Q exactive mass spectrometer operations course objective. Built with versatility in mind, the tripletof technology unleashes sensitivity, speed, and productivity in a single multifaceted platform to help you dig deeper into complex samples. Check voltage readbacks on analyzer power supplies.
The q exactive hf is only rarely reaching its max of 10 msms spectra per full ms spectrum while both q exactive hfx methods reach 12 and 18 msms spectra per full ms spectrum for the majority of the main gradient. Esi and apci source uhplc system ultimate 3000 dionex nanomate fraction collectorinjection robot advion available pdadetector thermo. The goal of the course is to provide you with a total solution for your analytical needs, with the focus on the use of thermo scientific q exactive high resolution lcms in forensic. Check ms1 calibration and transmission window using sliding window around infusion of known analyte. Exactive plus mass spectrometer are available as pdf files on the data system computer. The human sample will be extracted using a novel spe method as described in another poster. Mass spectrometry ms based proteomics aims at the comprehensive analysis of. Tune plus driver controls of the ma3100 are embedded within ms software package exactive plus, exactive plus emr, q exactive, and q exactive plus excellims visiontm control and visiontm analysis ma3100 control and data acquisition software for compatible mass spectrometers outside of the exactive product line excellims corporation. Q exactive plus, q exactive hf, tmt, proteome discoverer 2. Thermo scientific exactive plus benchtop fullscan orbitrap mass. The thermo scientific q exactive plus takes quanfirmation to the next level with increased performance and new. Genecraft labs trusted partner for laboratory solution. The superior quality of q exactive msms data provides ultimate confidence for a wide range of qualitative and quantitative applications. It is simple to use and maintenance on the q exactive is usually straightforward when outlined in the manual.
Maldi mass spectra were obtained on a bruker impacttm ii maldi mass spectrometer. Orbitrap qexactive plus mass spectrometer research. The q exactive plus lcmsms system not only offers broad screening capabilities but also excels at targeted quantitation experiments. In q exactive focus ms, can we collect data with one full. Lfquant is a new analysis tool for labelfree lcmsms quantitative proteomics data. Isobaric mass tagging quantification using q exactive. This guide is intended for all personnel who operate the q exactive mass spectrometer. You will receive a license file usually within 1 business day. The exactive plus tune software also provides help. Quantitative bioanalysis using the q exactive hrms.
Printed manuals exactive plus operating manual ion max and ion maxs api source hardware manual in addition to these printed manuals, other documents for the exactive plus mass spectrometer are available as pdf files on the data system computer. Preliminary data the q1 scan mass spectrums of single stranded phosphorothioate oligonucleotides 20mer, 19mer, 18mer and 14mer and a double stranded phosphodiester sirna 22mer were evaluated. Tmhe thero scientific q exactive plus benchtop lchrms combines highperformance quadrupole precursor selection with highresolution up to 280,000 fwhm accuratemass hram orbitrap detection to deliver high performance and tremendous versatility. Pdf introduction 120 words thermo scientific has recently introduced two new mass spectrometers ms. Thermo scientific q exactive plus thermo scientific q exactive hf. Thermo scientific q exactive orbitrap lcmsms system. Q exactive classicplus instruments were not tested and are not officially supported.
Enhanced analysis of native proteins and protein complexes with optional intact protein mode. Protein identification data dependent and data independent analyses. Exactive operating manual thermo fisher scientific. Overview purpose the gold standard for quantitative bioanalysis is a triple stage quadrupole ms operating under srm mode.
The q exactive mass spectrometer is superbly suited to untargeted or targeted screening with highconfidence confirmation, but is equally capable of a broad range of qualitative and quantitative applications. Mass spectrometrybased proteomics using q exactive, a. The q exactive hram instrument provides virtually no background noise resulting in excellent sn, low lloq. In contrast to other existing mzxml conversion utilities, rthur uses thermo foundation 3. This agent may also inhibit tumor angiogenesis by inhibiting endothelial. The qexactive plus is equipped with an easynlc proxeon and has been installed in 20142015. Performance evaluation of the q exactive hfx for shotgun. This technique requires tuning of each analytes and. Q exactive resolution at mz 200 free injection time ms approx. Normal mass range the msperformancemax ltq velos hybrid calmix, enables calibration of thermo scienti.
Overview to scale the picture below is a simulation at scale of the thermo q exactive lcms system spectrometer right side connected with a dionex uhplc left side, installed on a ionbench ms mass spec bench or table the vacuum pump leybold sv65 is enclosed in the noise reduction cabinet, under your. The q exactive hf, a benchtop mass spectrometer with a prefilter. The q exactive is an excellent system that generates reproducible, solid datasets. The program is specifically designed for use with orbitrap fusion data, and is also tested to be fully compatible with the ltq orbitrap and q exactive plus. Captopril is a sulfhydrylcontaining analog of proline with antihypertensive activity and potential antineoplastic activity. For information about performing these tasks, refer to the exactive plus operating manual, the exactive plus software manual or the exactive plus tune help, and the ion max and ion maxs api source hardware manual. The q exactive plus hybrid quadrupoleorbitrap mass spectrometer takes quanfirmation to the next level with increased performance and new options that enhance applications from confident dmpk qualquan screening studies to characterization of intact monoclonal antibodies. Scope of this guide the q exactive plus software manual includes the following chapters.
Dataindependent acquisition for the orbitrap q exactive. Alternatively, the q exactive hfx is capable of achieving the same proteome coverage as its predecessor in approximately half the gradient time or at 10fold lower sample loads. Who uses this guide this exactive operating manual is intended for all personnel that need a. I understand that i may be liable for a field service representatives travel or lodging expenses if they are una ble to carry out the installation on the pr escheduled date due to insufficient lab p reparation. Thermo scientific q exactive plus hybrid quadrupole. If necessary, create empty log and scanprotocols folders manually.
This guide is intended for all personnel who operate the q exactive plus mass spectrometer. The data system computer with the exactive plus tune software is switched on and the mass spectrometer is placed in standby mode. This q exactive plus quickstart guide gives an introduction on setting up and using the thermo scientific q exactive plus mass spectrometer. On instruments with a quadrupole as the first mass analyzer qqq, qtof, qorbitrap, the ms1 transmission window and calibration is often ignored but is critical for precursor ion selection. Q exactive plus orbitrap lcmsms system thermo fisher scientific. Accurate mass spectrometer to expand the scope of epa.
Monoclonal antibody characterization on q exactive and. Thermo scientific q exactive plus orbitrap lcmsms system. Targeted proteomic quantification on quadrupoleorbitrap. This exactive operating manual describes the modes of operation and principle hardware components of your exactive instrument. The results for the neat li sample are shown in figure. The q exactive hf mass spectrometer can be used to monitor six data points at a resolution setting of 60 k fwhm at mz 200, or 12. Q exactive plus ms, q exactive hf ms, q exactive hfx ms, biopharma option, monoclonal antibodies goal this document is intended to address the technical and workflow benefits of using the q exactive hfx mass spectrometer ms. View brochure download pdf file lr, 4mb download pdf file hr, 29mb office. Captopril competitively inhibits angiotensin converting enzyme ace, thereby decreasing levels of angiotensin ii, increasing plasma renin activity, and decreasing aldosterone secretion. Q exactive plus with ultrahigh field orbitrap analyzer. This q exactive plus software manual is intended for all personnel that need to perform measurements with the q exactive plus mass spectrometer, especially the key operator. To activate spectrodive using a license file, click on the rowse. The thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer combines highperformance quadrupole precursor selection with highresolution, accuratemass hram orbitrap detection. I am using a q exactive plus and in the experiment pane for a full ms ddms2 topn there are three settings for the automatic gain control agc, the number of ions to be in.
Ionbench with the thermo q exactive mass spectrometer. Q exactive mass spectrometer series calibration and. Tripletof 6600 high resolution accurate mass system sciex. This manual should be kept near the instrument to be available for quick reference. Lchrms for quantitative bioanalysis in the regulated. In addition, this manual provi des stepbystep instruct ions for cleaning and maintaining your instrument. Please note that this guide does not contain any safe ty notices and precautionary statements. Selecting the best q exactive orbitrap mass spectrometer. Then click on file export and give it a path and file name, this will save the template as a csv file.
Q beexactive, a nchtop quadrupole orbitrap mass spectrometer 3 mass spectrometrybased proteomics often involve the analysis of complex mixtures of proteins derived from cell or tissue lysates or from body fluids, posing tremendous analytical challenges. In addition to these printed manuals, other documents for the. It is compatible with highresolution mass spectrometers thermo raw data and two popular database search engines sequest and mascot with targetdecoy search strategy. The thermo scientific q exactive hf hybrid quadrupoleorbitrap mass spectrometer features an ultrahighfield orbitrap analyzer. The defining difference of the q exactive compared with the exactive is the presence of a mass selective quadrupole analyzer between the ion source and the ctrap fig.
The superior quality of q exactive plus msms data enables identification and quantitation. Q exactive plus hybrid quadrupoleorbitrap mass spectrometer. Used thermo q exactive mass spectrometer for sale dotmed. We use it in the lab to perform shotgun proteomic and metaproteomic analysis of biological specimens within various studies. Visit the planet orbitrap website for more information 111920 qe plus sn03039l installed 662012 qe sn01284l installed, 111420 retired scan rate. Tips for upgrading to win10 ltsb 2016 on tng instruments pdf. Up to 12 hz at resolution setting of 17,500 at mz 200. Exactive series installation request form please refer to the exactive series prei nstallation requirements guide pn 1288110, re vision a for the complete site requirem ents. You can access pdf files of the documents listed above and of this guide from the data system computer.
Calibrating thermo exactive with the direct analysis in. Monoclonal antibody characterization on q exactive and oribtrap elite yi zhang, ph. Thus, it is mandatory to read also the q exactive operating manual and the ion max and ion maxs api source hardware manual. The superior quality of q exactive data enables identification and quantitation of more compounds with greater confidence. Thermo scientific q exactive hf orbitrap lcmsms system. Bre0012255 revision a april 2017 exactive series exactive plus, exactive plus emr, q exactive, q exactive focus, q exactive plus, q exactive hf, and q exactive hfx. Identify, quantify and confirm more compounds rapidly and with. Unpack the zip file in a new folder on the local hard drive of the acquisition computer. Since the q exactive hf, operating with an ms2 resolution setting of 60000 performs the same as the q exactive plus ms, operating at 35000 and to demonstrate the versatility of our method, both samples were analyzed on the q exactive plus ms using 2 isolation widths 0. Q exactive q exactive plus creating solutions ltq velos hybrid calmix tfs210 for calibration mode.
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